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KMID : 1020220170170010013
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Journal of Korean Society of Dental Hygiene
2017 Volume.17 No. 1 p.13 ~ p.25
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Analysis of total oral microorganisms in saliva using real-time PCR and colony forming unit
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Yoo Su-Min
Jeong Seong-Kug
Yoo Hyun-Jun
Jang Jong-Hwa
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Abstract
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Objectives: The purpose of this study was to compare colony forming unit (CFU) method and multiplex real-time polymerase chain reaction (MRT-PCR) method for accurate quantitative analysis of bacteria.
Methods: We compared the CFU method and the MRT-PCR method, which are still used in Korea, for Prevotella intermedius (P. intermedius), a periodontal disease pathogen selected by MRT-PCR, and Streptococcus mutans (S. mutans), a dental caries causative organism. The subjects of this study were 30 patients who visited the C dental hospital.
Results: Total microorganisms in MRT-PCR method were significantly higher in both types of bacteria (p<0.05), since DNA of dead bacteria was also analyzed. This was because the periodontal dise(-) anaerobes, and even dead bacteria contain large amounts of toxic substances called LPS in the extracellular membrane, and fimbriae and pili, which are motility structures, still remain as a strong toxic substance in periodontal tissue.
Conclusions: Therefore, in terms of the total amount of bacteria found, the MRT-PCR method will be a useful technique for searching all the bacteria in the oral cavity including live bacteria, as well as sterilization.
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KEYWORD
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Colony forming unit , Multiplex real-time polymerase chain reaction (MRT-PCR) , Oral microorganisms , Saliva
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